Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: A high throughput flow cytometry assay for identification of inhibitors of 3’, 5’-cyclic adenosine monophosphate efflux

doi: 10.1007/978-1-4939-3673-1_15

Figure Lengend Snippet: Sample data from hit compound dose-response validation. A) Magnified time vs FL1-H plot for an F-cAMP efflux 8-point dose response for the hit compound of interest, clioquinol, including negative and positive controls. B) Graph of normalized response for the data shown in (A). Sample FL1-H MFI were normalized such that F-cAMP fluorescence at the time of compound addition = 100%. Data were fit by sigmoidal curves with Hill slope = 1 and top < 250. Graph, fit, and EC50 determination were done with GraphPad Prism 5.01 software (GraphPad Software, Inc., La Jolla, CA, USA).

Article Snippet: Graph, fit, and EC 50 determination were done with GraphPad Prism 5.01 software (GraphPad Software, Inc., La Jolla, CA, USA). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 6. caption a7 Results of ICE hit compounds after overnight incubation on U937 leukemia cell vitality in flow cytometric secondary assays.

Techniques: Fluorescence, Software